NEUTROPHIL TRANSFUSION - EFFECT OF STORAGE AND OF COLLECTION METHOD ON NEUTROPHIL BLOOD KINETICS

Google Scholar
  • 1 January 1978
    • journal article
    • research article
    • Vol. 51  (5) , 789-798
Abstract
The kinetics in blood of autologous neutrophils collected by phlebotomy, filtration leukapheresis (FL) or intermittent-flow centrifugation (IFC) and labeled with 32P-diisopropylfluorophosphate, and stored at 4.degree. C for up to 2 days were measured in 41 normal [human] subjects. Mean initial recovery for unstored IFC cells was 34.0%, compared to 7.9% for unstored FL cells. Blood half-lives were 4.1 and 2.7 h for unstored IFC and FL cells, respectively. With neutrophils collected by phlebotomy and stored in whole blood for 1-2 days, posttransfusion recoveries and blood half-times were significantly decreased. Storage of IFC and FL preparations resulted in only moderate kinetic abnormalities in comparison to the unstored cells. The ability of unstored IFC cells to circulate is probably basically normal; that of unstored FL cells is significantly impaired. These neutrophil concentrates probably should be stored for 1-2 days prior to transfusion.