Comparison of genomes of closely related phages ø29, ø15 and PZA using a rapid method of parallel physical mapping
- 19 August 1985
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 188 (1) , 123-126
- https://doi.org/10.1016/0014-5793(85)80887-5
Abstract
The DNAs of phages ø29, ø15 and PZA of Bacillus subtilis were analysed with restriction enzymes EcoRI, HpaI and HindIII. A method was used which permits parallel physical mapping of all three phages, from both ends of their linear genomes. The method is based on transfer of partially digested DNA to DBM paper and sequential hybridization with labelled terminal fragments. It follows from the comparison of the physical maps that phages ø29, ø15 and PZA are closely related and that they probably have arisen from a common ancestor by accumulation of point mutations.Keywords
This publication has 8 references indexed in Scilit:
- Initiation of phage phi 29 DNA replication in vitro: formation of a covalent complex between the terminal protein, p3, and 5'-dAMP.Proceedings of the National Academy of Sciences, 1982
- In vitro replication of bacteriophage phi 29 DNA.Proceedings of the National Academy of Sciences, 1982
- Restriction and modification in Bacillus subtilis 168Molecular Genetics and Genomics, 1982
- Nucleotide sequence of the major early region of bacteriophage φ29Gene, 1982
- [15] Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paperPublished by Elsevier ,1979
- Physical map of bacteriophage ∅29 DNAVirology, 1976
- A simple method for DNA restriction site mappingNucleic Acids Research, 1976
- Studies on Bacillus subtilis bacteriophage φ15Virology, 1973