Interaction of caldesmon with phospholipids
- 15 April 1993
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 291 (2) , 403-408
- https://doi.org/10.1042/bj2910403
Abstract
The interaction of caldesmon with liposomes composed of various phospholipids has been examined by tryptophan fluorescence spectroscopy. The results indicate that caldesmon makes its strongest complex with phosphatidylserine (PS) vesicles (Kass. = 1.45 x 10(5) M-1). Both electrostatic and hydrophobic interactions contribute to the stability of this complex. The site for strong binding of PS seems to be located in the N-terminal part of the 34 kDa C-terminal fragment of caldesmon. Binding of PS at this site results in displacement of calmodulin from its complex with caldesmon.Keywords
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