Measurement of scattering properties of individual particles with a scanning flow cytometer
- 20 September 1995
- journal article
- Published by Optica Publishing Group in Applied Optics
- Vol. 34 (27) , 6301-6305
- https://doi.org/10.1364/ao.34.006301
Abstract
A hydrofocusing head with an optical cuvette has been developed for the flow cytometer to generate complete scatter patterns of single particles at scattering angles ranging from 10° to 120°. The scatter signal has been measured as a function of the angle (a flying indicatrix) by the use of particle motion within a scanning system of the flow cytometer by the use of a single photomultiplier. Scattering data measured with the flow cytometer have been compared with those calculated from Mie theory for latex particles. A calculation algorithm has been used to estimate the size and the refractive index of spherical particles from the scattering data measured.Keywords
This publication has 3 references indexed in Scilit:
- Differential light scattering photometer for rapid analysis of single particles in flowApplied Optics, 1980
- A scanning diffractometer for the rapid analysis of microparticles and biological cellsJournal of Colloid and Interface Science, 1979
- Cell discrimination by multiangle light scattering.Journal of Histochemistry & Cytochemistry, 1976