Identification of Human Blood in Mosquitoes (Diptera: Culicidae) Using Nonradioactive DNA Dot Blot Hybridization
- 1 November 1992
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Medical Entomology
- Vol. 29 (6) , 1045-1048
- https://doi.org/10.1093/jmedent/29.6.1045
Abstract
A dot blot hybridization procedure was developed to detect human blood meals in engorged mosquitoes. A biotinylated DNA probe allowed the detection of 10–100 ng of human DNA, discriminated well between human and nonhuman sources of blood, and cross-reacted only with monkey DNA. Results showed that this method was a specific and sensitive technique for the identification of blood meals up to 100 h after ingestion. The nonisotopic label offers easy handling without the problems inherent in the use of radioisotopes, and it can be adapted for use in routine field tests.Keywords
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