Methylation of messenger RNA of Newcastle disease virus in vitro by a virion-associated enzyme.
- 1 July 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (7) , 2611-2615
- https://doi.org/10.1073/pnas.72.7.2611
Abstract
Purified Newcastle disease virus contains an enzyme that incorporates the methyl group from S-adenosyl-L-methionine into RNA synthesized in vitro by the virion-associated RNA polymerase (RNA nucleotidyltransferase). Incorporation of radioactivity from S-adenosyl-L-[methyl-3H]methionine was totally dependent upon RNA synthesis. The methylation reaction was completely inhibited by S-adenosyl-L-homocysteine, suggesting the transfer of only the methyl group of S-adenosyl-methionine to RNA products. Velocity sedimentation and hybridization of the in vitro product RNA indicated that both [3H]methyl and [32P]GMP labels resided in single-stranded 18S RNA molecules which were virus specific. Approximately 1 to 2 methyl groups were incorporated per RNA molecule. DEAE-cellulose chromatography of product RNA after alkaline hydrolysis suggested that the 5' terminus was the site of methylation.Keywords
This publication has 34 references indexed in Scilit:
- In vitro synthesis of methylated messenger RNA by the virion-associated RNA polymerase of vesicular stomatitis virusCell, 1974
- Methylated Messenger RNA Synthesis In Vitro by Purified ReovirusProceedings of the National Academy of Sciences, 1974
- Methylation of Newly Synthesized Viral Messenger RNA by an Enzyme in Vaccinia VirusProceedings of the National Academy of Sciences, 1974
- In vitro tnethylation of nascent reovirus mRNA by a virion-associated methyl transferaseNucleic Acids Research, 1974
- Identification of transcriptive and replicative intermediates in Sendai virus-infected cellsVirology, 1972
- Guanosine-5′-diphosphate at the 5′ termini of reovirus RNA: Evidence for a segmented genome within the virionJournal of Molecular Biology, 1971
- 2'-O-methyl polynucleotides as templates for cell-free amino acid incorporationBiochemistry, 1971
- Structure and Synthesis of Ribosomal RNAAnnual Review of Biochemistry, 1970
- ENZYMATIC METHYLATION OF RIBONUCLEIC ACID + DEOXYRIBONUCLEIC ACID .V. PURIFICATION + PROPERTIES OF DEOXYRIBONUCLEIC ACID-METHYLATING ACTIVITY OF ESCHERICHIA COLI1964
- Studies on mixed infection with Newcastle disease virusVirology, 1959