The Chemical Modification of the Lipase ofHumicola lanuginosaby N-Bromosuccinimide in Urea Solution
- 1 January 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 41 (1) , 131-135
- https://doi.org/10.1080/00021369.1977.10862451
Abstract
The chemical modifications of the lipase of Humicola lanuginosa by various reagents were investigated in the presence of urea. It was found that the lipase was completely inactivated by the addition of N-bromosuccinimide (NBS) in both the presence or absence of urea. According to the change of UV-spectra at 280 nm and 260 nm, it was confirmed that tryptophan residues of the Humicola lipase were specifically oxidized by N-bromourea which was produced by the reaction of NBS with urea. The lipase activity disappeared completely along with the oxidation of two of the three tryptophan residues in the lipase molecule.This publication has 6 references indexed in Scilit:
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