Identification of Corticotropin-Releasing Factor (CRF) Target Cells and Effects of Dexamethasone on Binding in Anterior Pituitary Using a Fluorescent Analog of CRF*
- 1 November 1986
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 119 (5) , 2376-2382
- https://doi.org/10.1210/endo-119-5-2376
Abstract
A fluorescein-conjugated bioactive analog of corticotropin-releasing factor (CRF) was synthesized and used to label cells that have high affinity CRF-binding sites. Of cultured bovine anterior pituitary cells, 6.1 .+-. 0.6% were visible by fluorescence microscopy after incubation with the analog. Fluorescence was eliminated by coincubation with a 200-fold excess of unlabeled CRF. Treatment with dexamethasone (10-9-10-7 M) decreased visible fluorescence in a dose-dependent manner. These results demonstrate the utility of a fluorescent CRF analog for identification of cells with specific CRF-binding sites and suggest that binding of CRF to anterior pituitary cells is altered by glucocorticoids.This publication has 13 references indexed in Scilit:
- Synthetic Competitive Antagonists of Corticotropin-Releasing Factor: Effect on ACTH Secretion in the RatScience, 1984
- Radioautographic Study of Binding and Internalization of Corticotropin-Releasing Factor by Rat Anterior Pituitary CorticotrophsEndocrinology, 1984
- Effects of Synthetic Ovine Corticotropin-Releasing Factor, Glucocorticoids, Catecholamines, Neurohypophysial Peptides, and Other Substances on Cultured Corticotropic Cells*Endocrinology, 1983
- Glucocorticoids Inhibit Corticotropin-Releasing Factor-Induced Production of Adenosine 3′,5′-Monophosphate in Cultured Anterior Pituitary Cells*Endocrinology, 1983
- Stimulation of cyclic AMP accumulation and corticotropin release by synthetic ovine corticotropin-releasing factor in rat anterior pituitary cells: site of glucocorticoid action.Proceedings of the National Academy of Sciences, 1982
- Inhibition of β-Adrenergic Responsiveness in Muscle Cell Cultures by Dexamethasone*Endocrinology, 1981
- Rapid glucocorticoid inhibition of vasoactive intestinal peptide-induced cyclic AMP accumulation and prolactin release in rat pituitary cells in culture.Proceedings of the National Academy of Sciences, 1981
- Characterization of a 41-Residue Ovine Hypothalamic Peptide That Stimulates Secretion of Corticotropin and β-EndorphinScience, 1981
- Interaction of fluorescent gonadotropin-releasing hormone with receptors in cultured pituitary cells.Journal of Biological Chemistry, 1981
- Receptor-mediated internalization of fluorescent gonadotropin-releasing hormone by pituitary gonadotropes.Proceedings of the National Academy of Sciences, 1980