Dithiols simulate endotoxin in theLimulus reaction
- 1 September 1978
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 34 (9) , 1154-1155
- https://doi.org/10.1007/bf01922927
Abstract
Dithiothreitol, dithioerythritol and bacterial lipopolysaccharides increase optical absorbance and clotLimulus lysate. Purification of dithiothreitol from possible endotoxin contamination by vacuum sublimation or chromatography does not abolish the reaction with lysate. The dithiols reported active here represent the smallest molecules capable of simulating endotoxin in theLimulus test.Keywords
This publication has 14 references indexed in Scilit:
- A sensitive spectrophotometric method for measurement of plasma endotoxinBiochemical Medicine, 1976
- Biology of EndotoxinAnnual Review of Medicine, 1976
- Purification and properties of the clotting enzyme from limulus lysateBiochemical and Biophysical Research Communications, 1975
- Rapid Detection of Gram-Negative Bacterial Meningitis by the Limulus Lysate TestNew England Journal of Medicine, 1973
- Nonspecificity of the Limulus Amebocyte Lysate Test: Positive Reactions with Polynucleotides and ProteinsThe Journal of Infectious Diseases, 1973
- The coagulogen of Limulus polyphemus hemocytes. A comparison of the clotted and non-clotted forms of the moleculeThrombosis Research, 1973
- An invertebrate coagulation system activated by endotoxin: evidence for enzymatic mediationJournal of Clinical Investigation, 1972
- Picogram-sensitive assay for endotoxin: Gelation of Limulus polyphemus blood cell lysate induced lipopolysaccharides and lipid A from gram-negative bacteriaBiochimica et Biophysica Acta (BBA) - General Subjects, 1972
- Gram-Negative Sepsis: Detection of Endotoxemia with the Limulus TestAnnals of Internal Medicine, 1972
- Dithiothreitol, a New Protective Reagent for SH Groups*Biochemistry, 1964