Inhibitory Effects of Substrate Analogs on Lactate Production from Fructose and Glucose in Bovine Spermatozoa1

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Abstract
A number of substrate analogs were tested as inhibitors of bovine sperm lactate formation from glucose and from fructose. When 1 mM glucose was the substrate, lactate production was inhibited by 5-thioglucose, 2-deoxyglucose, 2,5-anhydromannitol, and 5-ketofructose while no inhibition was observed with 1-deoxyfructose, 4,6-ethylideneglucose, N-acetylglucosamine, or 3-0 methylglucose at concentrations up to 10 mM. 5-Thioglucose was most effective, producing complete inhibition at a 10 mM concentration. Inhibition by the other inhibitory analogs was not complete at 10 mM. All of the observed inhibitions could be overcome by increasing glucose concentration to 10 mM.