Uptake of metabolites by germinating blastospores ofHistoplasma capsulatum

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Abstract
The uptake of radioactive L-leucine, L-glutamic acid and guanine was monitored during blastospore germination of H. capsulatum. Blastospores of H. capsulatum, grown in Salvin''s medium at 37.degree. C for 48 h and shifted to 23.degree. C, germinated within 24 h. Metabolite uptake was faster in mycelial cells at 23.degree. C than it was in yeast cells at 37.degree. C. During the yeast to mold transition, the initial rate of uptake was first markedly depressed and then increased and achieved a level characteristic of the mycelial phase of growth. Estimates of macromolecular synthesis occurring during transitional morphological events might be influenced by these differences in the rate of uptake.