INTERACTION OF CYTOCHROME-C WITH CYTOCHROME-C OXIDASE - PHOTOAFFINITY LABELING OF BEEF-HEART CYTOCHROME-C OXIDASE WITH ARYLAZIDOCYTOCHROME-C

  • 1 January 1978
    • journal article
    • research article
    • Vol. 253  (6) , 1874-1880
Abstract
Cytochrome c derivatives labeled with a 3-nitrophenylazido group at lysine 13, at lysine 22, or at both residues were prepared. The interaction of the cytochrome c derivatives with beef heart cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase, EC 1.9.3.1) in the presence of UV light results in formation of a covalent complex between cytochrome c and the oxidase. Using the lysine 22 derivative, the polypeptide composition of the oxidase is not modified, nor is its catalytic activity, whereas with the lysine 13 derivative, the gel electrophoretic pattern is altered and the catalytic activity of the complex diminished. The data are consistent with a specific covalent interaction of the lysine 13 derivative of cytochrome c with the polypeptide of MW 23,700 (Subunit II) of cytochrome c oxidase.

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