The Effect of Composition of the Atmosphere and Duration of Exposure on Brown Stain of Lettuce1,2

Abstract
The severity of brown stain on crisphead lettuce (Lactuca sativa L., cvs. Calmar and Great Lakes 118) held at 0° or 2.5°C for 10 days increased with increasing CO2 (1 to 5%) and decreasing O2 (21 to 1%) levels. Very slight or no brown stain developed on lettuce subjected to 0, 1, or 2% CO2 in combination with 10 or 21% O2 for 10 days or on that held in 2.5 or 5% CO2 + 2% O2 for 4 days at 2.5°C. In 2.5 or 5% CO2 + 2% O2, brown stain intensified with duration of exposure (2 to 30 days) at 0° or 2.5°C.

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