INTERACTION OF [C-14]ACETYLSALICYLIC ACID WITH NORMAL HUMAN PERIPHERAL-BLOOD LYMPHOCYTES

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 31  (3) , 482-489
Abstract
Therapeutic concentrations of acetylsalicylic acid (ASA) have strikingly inhibited in vitro and in vivo mitogen- and antigen-induced blastogenesis by human lymphocytes. These observations may be pertinent to the anti-inflammatory actions of ASA. To further investigate the possible effects of ASA on cellular responses, the in vitro interaction of [14C]ASA with lymphocytes was studied. The [14C]ASA association with cells was apparently proportional to ASA concentrations, non-saturable at high concentrations of ASA, dependent on pH, independent of temperature, dependent on cell concentration, non consistently displaced by unlabeled ASA or other drugs, rapid and unchanged over 1 min to 72 h incubations and was reversed by repeated cell washing. Thus, ASA interacted with lymphocytes. The association was rapid, reversible, pH-dependent and not demonstrably specific under these experimental conditions.