Construction and Properties of Hybrids Obtained in Interspecific Crosses between Streptomyces coelicolor A3(2) and Streptomyces griseus Kr. 15

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Abstract
Recombinants between S. coelicolor A3(2) and S. griseus Kr.15 were obtained using methods of hybrid construction. Recombinant Rcg1, obtained from a cross between S. griseus and a S. coelicolor UF (SCP1-) strain, phenotypically resembled S. coelicolor UF strains and in crosses with a S. coelicolor NF donor strain produced recombinant progeny at a frequency of 100%. Recombinant Rcg3, like SCP1-carrying S. coelicolor strains, inhibited SCP1-strains of S. coelicolor and in crosses with a UF recipient strain of S. coelicolor generated recombinants at high frequency. In crosses between S. griseus and Rcg1 the frequency of recombinant formation was increased about 100-fold relative to crosses between S. griseus and S. coelicolor. Effective transfer of S. griseus and Rcg3 chromosomal markers into Rcg1 and S. coelicolor, respectively, indicated that S. griseus had donor properties. Studies of the ability of recombinants to support phage growth indicated that parental chromosomal fragments containing genes involved in control of phage-receptor formation and intracellular growth were present in the hybrids. Grisin-producing recombinants, capable of restricting phages attacking S. coelicolor and S. griseus, were obtained.