Virus-Free Shoots from Cassava Stem Cuttings Infected with Cassava Latent Virus

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Abstract
Cassava stem cuttings infected with cassava latent virus (CLV) gave rise to shoots that showed CLV symptoms at emergence, shoots that showed CLV symptoms 3-5 wk later and shoots that remained symptomless after 6 wk of observation. CLV symptoms appeared 5-11 days earlier when symptomless shoots were detopped than when they were left intact. Symptomless shoots severed 2 wk after emergence from CLV-infected stem cuttings and subsequently detopped did not develop CLV symptoms. These plants readily developed symptoms of CLV when colonized with CLV-laden Bemisia tabaci. Therefore, symptomless shoots were considered free of CLV and could be used as healthy planting stock.

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