The tale of two RNA polymerases: transcription profiling and gene expression strategy of bacteriophage Xp10
Open Access
- 9 December 2004
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 55 (3) , 764-777
- https://doi.org/10.1111/j.1365-2958.2004.04442.x
Abstract
Summary: Bacteriophage Xp10 infects rice pathogen Xanthomonas oryzae. Xp10 encodes its own single‐subunit RNA polymerase (RNAP), similar to that found in phages of the T7 family. On the other hand, most of Xp10 genes are organized in a manner typical of lambdoid phages that are known to rely only on host RNAP for their development. To better understand the temporal pattern of viral transcription during Xp10 development, we performed global transcription profiling, primer extension, chemical kinetic modelling and bioinformatic analyses of Xp10 gene expression. Our results indicate that true to its mosaic nature, Xp10 relies on both host and viral RNAPs for expression of genes coding for virion components and host lysis. The joint transcription of the same set of genes by two types of RNA polymerases is unprecedented for a bacteriophage. Curiously, such a situation is realized in chloroplasts.Keywords
This publication has 14 references indexed in Scilit:
- A Biophysical Approach to Transcription Factor Binding Site DiscoveryGenome Research, 2003
- Phage N4 RNA polymerase II recruitment to DNA by a single-stranded DNA-binding proteinGenes & Development, 2003
- Genome of Xanthomonas oryzae Bacteriophage Xp10: An Odd T-Odd PhageJournal of Molecular Biology, 2003
- A Novel Bacteriophage-encoded RNA Polymerase Binding Protein Inhibits Transcription Initiation and Abolishes Transcription Termination by Host RNA PolymeraseJournal of Molecular Biology, 2002
- Prediction of transcription terminators in bacterial genomes 1 1Edited by F. E. CohenJournal of Molecular Biology, 2000
- Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structureJournal of Molecular Biology, 1999
- Antitermination by Bacteriophage Q ProteinCold Spring Harbor Symposia on Quantitative Biology, 1998
- Sensitive detection of bacterial transcription initiation sites and differentiation from RNA processing sites in the pheromone-induced plasmid transfer system of Enterococcus faecalis.Proceedings of the National Academy of Sciences, 1996
- Detecting Subtle Sequence Signals: a Gibbs Sampling Strategy for Multiple AlignmentScience, 1993
- Loss of sigma-factor of RNA polymerase of Xanthomonas campestris pv. oryzae during phage Xp10 infection.Journal of Biological Chemistry, 1986