Cloning and Expression of cDNAs from Enterically‐Transmitted Non‐A, Non‐B Hepatitis Virus
Open Access
- 1 July 1991
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 35 (7) , 535-543
- https://doi.org/10.1111/j.1348-0421.1991.tb01584.x
Abstract
The fragment gene of enterically‐transmitted non‐A, non‐B hepatitis virus (ET‐NANBHV) was cloned as a cDNA and inserted into an expression vector pUEX2. The recombinant protein was expressed in Escherichia coli HB101 as a fusion protein with β‐galactosidase (β‐Gal). The fusion protein reacted with the sera of infected cynomolgus monkeys and of patients from Myanmar. This reaction was highly related with ET‐NANBHV infection, and obviously demonstrates in that the recombinant protein can be used for the detection of ET‐NANBHV infection.Keywords
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