Association of the lupus antigen La with a subset of U6 snRNA molecules
Open Access
- 1 January 1985
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 13 (7) , 2617-2629
- https://doi.org/10.1093/nar/13.7.2617
Abstract
U6 snRNA is a component of the major class of small RNA-protein complexes, the Sm snRNPs, present in mammalian cell nuclei. Here we report that a substantial fraction (about 10%) of U6 RNA from human and mouse cells is associated with another lupus antigen, the 50 kd La protein. The La-bound U6 subpopulation is characterized by 3' end heterogeneity and partial undermethylation. These U6 molecules have U-rich 3' termini that could be responsible for their selective association with the La protein. The question of whether they are precursors to the major U6 RNAs found in Sm snRNPs is discussed.Keywords
This publication has 43 references indexed in Scilit:
- Purified lupus antigen la recognizes an oligouridylate stretch common to the 3′ termini of RNA polymerase III transcriptsCell, 1984
- The leader RNA of vesicular stomatitis virus is bound by a cellular protein reactive with anti-La lupus antibodiesCell, 1983
- Genes for two small cytoplasmic Ro RNAs are adjacent and appear to be single-copy in the human genomeCell, 1983
- Structural organization of ribonucleoproteins containing small nuclear RNAs from HeLa cellsJournal of Molecular Biology, 1982
- Human 7SL RNA consists of a 140 nucleotide middle-repetitive sequence inserted in an Alu sequenceCell, 1982
- The nucleotide sequence of nuclear 4.8S RNA of mouse cellsBiochemical and Biophysical Research Communications, 1980
- Subsets in Systemic Lupus ErythematosusJournal of Investigative Dermatology, 1979
- Low molecular weight RNAs hydrogen-bonded to nuclear and cytoplasmic poly(a)-terminated RNA from cultured chinese hamster ovary cellsCell, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977