Endocytosis and inositol hexakisphosphate levels inras transformants ofDictyostelium discoideum amoebae
- 1 April 1989
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 45 (4) , 365-367
- https://doi.org/10.1007/bf01957480
Abstract
Fluid-phase pinocytosis kinetics and lysosomal enzyme secretion parameters were measured inDictyostelium discoideum amoebae constructed from strain AX3 by transformation with a multicopy plasmid carrying either a normalras gene (ras-Gly12), a mutatedras gene (ras-Thr12) or by the vector carrying the geneticin resistance gene only (pDNEO2). It was found that the pinocytosis rate and extent as well as the lysosomal enzyme secretion were slightly different in the three strains. These changes, however, were related to minor modifications of the cellular volumes. The overall concentration of inositol hexakisphosphate was similar in the three strains.This publication has 24 references indexed in Scilit:
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