The effects of x-irradiation on the incorporation of amino acids into proteins of nuclei from rat thymus gland

Publisher Website
Google Scholar
Abstract
Protein synthesis in nuclei was inhibited by the addition of ATP. Damage to the nuclear structure occurred and the intranuclear thiol content was decreased. Enhanced protein synthesis was caused by reducing agents. Reagents that oxidized or reacted with thiol groups were inhibitory. Irradiation of isolated nuclei inhibited incorporation of amino acids into proteins. Nuclear protein synthesis was stimulated immediately after whole-body X-irradiation. Nuclei prepared from adrenalectomized rats reacted differently on irradiation and showed a decreased synthesis. Protein synthesis was inhibited in both instances 2 hr after irradiation.