MEMBRANE POTENTIAL AND RESISTANCE CHANGES IN NG108-15 CELLS: AN IN VITRO MODEL TO STUDY MEMBRANE-ACTIVE COMPOUNDS
- 1 January 1999
- journal article
- research article
- Published by Taylor & Francis in Toxicology Mechanisms and Methods
- Vol. 9 (1) , 35-45
- https://doi.org/10.1080/105172399242816
Abstract
The objective of this work was to develop an in vitro model to screen possible antidotal treatments against membrane-active compounds. Toward this end, studies were conducted to examine changes in membrane potential and resistance induced by several test compounds, i.e., gramicidin D, ouabain, and minoxidil, in differentiated NG108-15 hybrid (mouse neuroblastoma X rat glioma) cells using intracellular electrodes and standard current clamp techniques. In general, changes observed were consistent with known actions of these compounds and their effects demonstrated in a number ofother systems.For example, the channel-forming ionophore gramicidin D produced severe membrane depolarization with markedly reduced resistance. Also, the Na+-K+ pump blocker ouabain produced membrane depolarization, and the potassium channel activator minoxidil produced membrane hyperpolarization. Neither ouabain nor minoxidil significantly altered input resistanceof theNG108-15 cells.Thenature of theobserved alterations combined with theconsistency apparent in concentration-response profiles suggests that this model system is suitable for screening the effects of possible antidotal treatments against these and other membraneactive compounds.Keywords
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