The cosubstrate NADP(H) protects lysine 601 in the porcine NADPH‐cytochrome P‐450 reductase against pyridoxylation
- 29 January 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 260 (2) , 289-290
- https://doi.org/10.1016/0014-5793(90)80125-3
Abstract
Lys601 in NADPH-cytochrome P-450 reductase is modified by reductive alkylation with pyridoxal 5'-phosphate (pyridoxylation). Lys601 is protected against modification by the cosubstrate NADP(H).Keywords
This publication has 13 references indexed in Scilit:
- Coding Nucleotide, 5′ Regulatory, and Deduced Amino Acid Sequences of P-450BM-3, a Single Peptide Cytochrome P-450:NADPH-P-450 Reductase from Bacillus megateriumJournal of Biological Chemistry, 1989
- Affinity modification of NADPH-cytochrome P-450 reductaseBiochemical and Biophysical Research Communications, 1988
- Complete structure of the hydrophilic domain in the porcine NADPH-cytochrome P-450 reductaseBiochemical Journal, 1986
- NADPH-cytochrome P-450 oxidoreductase: flavin mononucleotide and flavin adenine dinucleotide domains evolved from different flavoproteinsBiochemistry, 1986
- Chemical modification of NADPH‐cytochrome P‐450 reductaseEuropean Journal of Biochemistry, 1986
- Isolation and sequencing of an active-site peptide from spinach ferredoxin-NADP+ oxidoreductase after affinity labeling with periodate-oxidized NADP+Archives of Biochemistry and Biophysics, 1985
- NADPH-Cytochrome P-450 Reductase (Pig Liver). Studies on the Sequence of the Cyanogen Bromide Peptides from the Catalytic Domain and on the Reactivity of the Thiol GroupsBiological Chemistry Hoppe-Seyler, 1985
- Structural analysis of NADPH-cytochrome P-450 reductase from porcine hepatic microsomes. Sequences of proteolytic fragments, cysteine-containing peptides, and a NADPH-protected cysteine peptide.Journal of Biological Chemistry, 1984
- Fluorescence labelling of NADPH-cytochrome P-450 reductase with the monobromomethyl derivative of syn-9,10-dioxabimaneBiochemical Journal, 1983
- STUDIES ON THE COSUBSTRATE SITE OF PROTEASE SOLUBILIZED NADPH‐CYTOCHROME P450 REDUCTASEInternational Journal of Peptide and Protein Research, 1980