Alterations of the p16 (MTS1) Gene in Testicular, Ovarian, and Endometrial Malignancies
- 1 November 1995
- journal article
- Published by Wolters Kluwer Health in Journal of Urology
- Vol. 154 (5) , 1954-1957
- https://doi.org/10.1016/s0022-5347(01)66833-2
Abstract
The p16 (MTS1) gene codes for a cyclin-dependent kinase inhibitor and may be a new tumor suppressor gene. It is frequently mutated in a variety of cell lines established from tumors. This is the first report of screening for alteration of the p16 gene in testicular, ovarian and endometrial malignancies.We examined alterations of p16 in 78 primary genital tumors (42 testicular, 21 ovarian and 15 endometrial cancers) and mononuclear cells from 2 patients with Lynch syndrome II as well as 5 testicular tumor cell lines by single-strand conformation polymorphism (SSCP) and Southern blot hybridization.The DNA from the p16 gene of 2 testicular tumors (5%), an ovarian cancer (4%) and a testicular tumor cell line (20%) had altered migration in gel electrophoresis as shown by SSCP. Analysis of DNA sequence of these samples revealed a polymorphism at codon 140. Southern blot hybridization detected neither deletions nor rearrangements of the p16 gene in any of the samples.Taken together, these results suggest that p16 alterations probably are not important for tumorigenesis of testicular, ovarian and endometrial tumors.Keywords
This publication has 31 references indexed in Scilit:
- Recurrent Cytogenetic Aberrations and Loss of Constitutional Heterozygosity in Ovarian CarcinomasGynecologic Oncology, 1994
- Molecular analysis of the retinoblastoma gene in primary ovarian cancer cellsInternational Journal of Cancer, 1994
- Somatic Mutations of the MTS (Multiple Tumor Suppressor) 1/CDK41 (Cyclin-Dependent Kinase-4 Inhibitor) Gene in Human Primary Non-small Cell Lung CarcinomasBiochemical and Biophysical Research Communications, 1994
- P16 gene in uncultured tumoursNature, 1994
- A Cell Cycle Regulator Potentially Involved in Genesis of Many Tumor TypesScience, 1994
- Deletions of the cyclin-dependent kinase-4 inhibitor gene in multiple human cancersNature, 1994
- A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4Nature, 1993
- Mapping of the shortest region of overlap of deletions of the short arm of chromosome 9 associated with human neoplasiaGenomics, 1992
- p53 Mutations in Human CancersScience, 1991
- Mutations in the p53 gene occur in diverse human tumour typesNature, 1989