NEUTROPHIL GRANULOCYTE CHEMOTAXIS INVITRO - COMPARISON OF RESPONSE TO CASEIN AND A BACTERIAL CHEMOTACTIC FACTOR, AND EVALUATION OF AN AUTOMATIC METHOD FOR COUNTING CELLS ON A MEMBRANE-FILTER SURFACE

Google Scholar
  • 1 January 1977
    • journal article
    • research article
    • Vol. 85  (4) , 289-296
Abstract
A method is described for evaluating neutrophil granulocyte chemotaxis in vitro using a modified reversible Boyden chamber. The variation in cell detachment from the attractant surface of the filter after the migration through the filter was apparently eliminated by reversing the chambers during the incubation period. In unreversed chambers, loss of cells from the bottom surface of the filter was much higher on attraction with casein than with a bacterial chemotactic factor (BCF) [from Escherichia coli]. The finding of a different kinetic response to casein and to BCF may suggest differing affinity to various chemoattractants within circulating neutrophils. The results obtained after introducing an automatic quantitative image analysis system for the counting of the cells at the filter surface are apparently well correlated to those with conventional direct microscope counting.

This publication has 1 reference indexed in Scilit: