FGF Binding and FGF Receptor Activation by Synthetic Heparan-Derived Di- and Trisaccharides
- 21 April 1995
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 268 (5209) , 432-436
- https://doi.org/10.1126/science.7536345
Abstract
Fibroblast growth factors (FGFs) require a polysaccharide cofactor, heparin or heparan sulfate (HS), for receptor binding and activation. To probe the molecular mechanism by which heparin or HS (heparin/HS) activates FGF, small nonsulfated oligosaccharides found within heparin/HS were assayed for activity. These synthetic and isomerically pure compounds can activate the FGF signaling pathway. The crystal structures of complexes between FGF and these heparin/HS oligosaccharides reveal several binding sites on FGF and constrain possible mechanisms by which heparin/HS can activate the FGF receptor. These studies establish a framework for the molecular design of compounds capable of modulating FGF activity.Keywords
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