Distribution of a putative cell surface receptor for fibronectin and laminin in the avian embryo.
Open Access
- 1 September 1986
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 103 (3) , 1061-1071
- https://doi.org/10.1083/jcb.103.3.1061
Abstract
The cell substratum attachment (CSAT) antibody recognizes a 140-kD cell surface receptor complex involved in adhesion to fibronectin (FN) and laminin (LM) (Horwitz, A., K. Duggan, R. Greggs, C. Decker, and C. Buck, 1985, J. Cell Biol., 101:2134-2144). Here, we describe the distribution of the CSAT antigen along with FN and LM in the early avian embryo. At the light microscopic level, the staining patterns for the CSAT receptor and the extracellular matrix molecules to which it binds were largely codistributed. The CSAT antigen was observed on numerous tissues during gastrulation, neurulation, and neural crest migration: for example, the surface of neural crest cells and the basal surface of epithelial tissues such as the ectoderm, neural tube, notochord, and dermomyotome. FN and LM immunoreactivity was observed in the basement membranes surrounding many of these epithelial tissues, as well as around the otic and optic vesicles. In addition, the pathways followed by cranial neural crest cells were lined with FN and LM. In the trunk region, FN and LM were observed surrounding a subpopulation of neural crest cells. However, neither molecule exhibited the selective distribution pattern necessary for a guiding role in trunk neural crest migration. The levels of CSAT, FN, and LM are dynamic in the embryo, perhaps reflecting that the balance of surface-substratum adhesions contributes to initiation, migration, and localization of some neural crest cell populations.This publication has 52 references indexed in Scilit:
- Analysis of the early stages of trunk neural crest migration in avian embryos using monoclonal antibody HNK-1Developmental Biology, 1986
- Distribution of laminin in the developing peripheral nervous system of the chickDevelopmental Biology, 1986
- Selective Inhibition of Fibronectin-Mediated Cell Adhesion by Monoclonal Antibodies to a Cell-Surface GlycoproteinScience, 1985
- Extracellular matrix organization in developing muscle: correlation with acetylcholine receptor aggregates.The Journal of cell biology, 1984
- Morphology and behavior of quail neural crest cells in artificial three-dimensional extracellular matricesDevelopmental Biology, 1984
- Pathways and mechanisms of avian trunk neural crest cell migration and localizationDevelopmental Biology, 1982
- Distribution of fibronectin in the early phase of avian cephalic neural crest cell migrationDevelopmental Biology, 1982
- Interaction of embryonic corneal epithelium with exogenous collagen, laminin, and fibronectin: Role of endogenous protein synthesisDevelopmental Biology, 1982
- Monoclonal Antibodies Which Alter the Morphology of Cultured Chick Myogenic CellsJournal of Cellular Biochemistry, 1982
- Integral Membrane Glycoproteins Related to Cell–Substratum Adhesion in Mammalian CellsJournal of Cellular Biochemistry, 1982