Identification by cross‐linking of a neuronal acceptor protein for dendrotoxin, a convulsant polypeptide
- 20 August 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 174 (1) , 116-122
- https://doi.org/10.1016/0014-5793(84)81088-1
Abstract
Dendrotoxin, a lijow molecular weight protein from the venom of Dendroaspis angusticeps, is known to be a potent convulsant that attenuates one type of voltage-sensitive K+ channel in guinea-pig hippocampus. A biologically active preparation of 125I-labelled dendrotoxin has been cross-linked to its high-affinity protein acceptor in synaptic plasma membranes from rat cerebral cortex. On SDS gel electrophoresis, a complex with a Mr of 72,000 was observed which, assuming one toxin molecule is attached, yields an apparent size of 65,000 for this subunit of the acceptor. Unlike dendrotoxin, low concentrations of β-bungarotoxin, another pre-synaptically acitve toxin, do not inhibit its labelling.Keywords
This publication has 16 references indexed in Scilit:
- Preparation of Neurotoxic 3H-β-Bungarotoxin: Demonstration of Saturable Binding to Brain Synapses and Its Inhibition by Toxin IEuropean Journal of Biochemistry, 2005
- Photoaffinity labeling of α- and β- scorpion toxin receptors associated with rat brain sodium channelBiochemical and Biophysical Research Communications, 1983
- Radioiodination of Botulinum Neurotoxin Type A with Retention of Biological Activity and Its Binding to Brain SynaptosomesEuropean Journal of Biochemistry, 1983
- Synaptic binding sites in brain for [3H]β-bungarotoxin — A specific probe that perturbs transmitter releaseNeurochemistry International, 1983
- The emerging molecular view of the sodium channelTrends in Neurosciences, 1982
- PROTEASE INHIBITOR HOMOLOGUES FROM MAMBA VENOMS: FACILITATION OF ACETYLCHOLINE RELEASE AND INTERACTIONS WITH PREJUNCTIONAL BLOCKING TOXINSBritish Journal of Pharmacology, 1982
- A transient outward current in a mammalian central neurone blocked by 4-aminopyridineNature, 1982
- Identification of a protein component of the Ca2+-dependent K+ channel by affinity labelling with apaminBiochemical and Biophysical Research Communications, 1982
- Apamin blocks certain neurotransmitter-induced increases in potassium permeabilityNature, 1979
- Isolation of synaptic plasma membrane from brain by combined flotation-sedimentation density gradient centrifugationBiochimica et Biophysica Acta (BBA) - Biomembranes, 1974