Growth monitoring and control in complex medium: A case study employing fed-batch penicillin fermentation and computer-aided on-line mass balancing
- 1 January 1983
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 25 (1) , 257-269
- https://doi.org/10.1002/bit.260250119
Abstract
To broaden the practicality of on-line growth monitoring and control, its application in fedbatch penicillin fermentation using high corn steep liquor (CSL) concentration (53 g/L) is demonstrated. By employing a calculation method that considers the vagaries of CSL consumption, overall and instantaneous carbon-balancing equations are successfully used to calculate, on-line, the cell concentration and instantaneous specific growth rate in the penicillin production phase. As a consequence, these equations, together with a feedback control strategy, enable the computer control of glucose feed and maintenance of the preselected production-phase growth rate with error less than 0.002 h−1.Keywords
This publication has 1 reference indexed in Scilit:
- Growth monitoring and control through computer-aided on-line mass balancing in a fed-batch penicillin fermentationBiotechnology & Bioengineering, 1983