Canine Limbic System Substance P Receptors

Abstract
A canine limbic system preparation is utilized as a source of substance P receptor(s) to screen gradient RP-HPLC fractions for the presence of receptoractive-substance P activity, and to quantify endogenous receptoractive-substance p in biological extracts such as human tooth pulp. The binding characteristics, KD = 1.3 nM and Bmax = 11 fmol mg-1 protein, are similar to values obtained from receptors produced from other biological sources such as whole rat brain, minus cerebellum.