DEVELOPMENT AND CHARACTERIZATION OF A MONOCLONAL ANTIBODY WHICH DISTINGUISHES THE β-SUBUNIT OF HUMAN CHORIONIC GONADOTROPIN (βhCG) IN THE PRESENCE OF THE hCG

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Abstract
A monoclonal antibody specific for the .beta.-subunit of human chorionic gonadotropin (.beta.hCG) was developed which has a cross-reactivity of 0.23% with intact hCG. There was no cross-reactivity with other glycoprotein pituitary hormones in the range in which they might be expected to be present in serum. The .beta.-subunits of hFSH, hTSH and human luteinizing hormone did not inhibit binding of [125I]iodo-.beta.hCG to the antibody at up to 250 ng/tube. The Kd with [125I]iodo-.beta.hCG was 3.3 .times. 10-10, and the reaction had reached equilibrium within 1 h.