Measurement of Plasma Volume in the Dog With High Concentrations of T-1824

Publisher Website
Google Scholar
Abstract
Twelve out of 16 paired successive estimates of plasma volume, 1 hr. apart, show that the volume distribution of T-1824 is independent of the quantity injected up to plasma concns. of 1.3 mg./ml. Extensive hemolysis occurred in 4 expts. where the plasma dye concn. exceeded 1.3 mg./ml. Plasma dye concns. were estimated with the paper pulp extraction procedure. The hemolytic effect of T-1824 occurs in vitro as well as in vivo. However, washed blood cells are 50% destroyed by only 0.02 mg./ml., which corresponds to the free dye in plasma when the total dye is 3 mg./ml. The human red cell is extremely resistant and either in plasma or saline is not affected by 4 mg./ml. The species difference suggests that the red cell membranes contain differing proteins. Toxic effects of the dye in dogs appear to be related to the occurrence of hemolysis.

This publication has 0 references indexed in Scilit: