Collagenase Digestion for Distinguishing Neural from Reticular Fibres in Silver Stains

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Abstract
Fresh frozen sections of liver and duck salt gland, 20 u thick were attached to slides and immersed for 20 min, in Carnoy''s 63:1 fixative; washed 3 times with 0.9% NaCl; placed in M/15 phosphate buffer, pH 7.0 for 20 min; then incubated for 5 hr. at 37[degree]C in a 0.1% solution of collagenase (Koch-Light Laboratories) in the phosphate buffer. After washing in 0.9% NaCl the slides were immersed for at least 24 hr. in 4% formaldehyde (10% formol- saline). Slides were examined for morphological detail after haematoxylin and eosin staining, for nerve fibers after silver impregnation, and for connective tissue fibers. Attempts to use papain and pepsin digestion on sections after similar fixation were not successful, as much of the tissue was destroyed.

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