High-Performance Liquid Chromatographic Separation of 2-Hydroxy Fatty Acid Enantiomers on a Chiral Slurry- Packed Capillary Column
- 1 October 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 27 (10) , 574-577
- https://doi.org/10.1093/chromsci/27.10.574
Abstract
Complete enantiomer separations of racemic 2-hydroxy fatty acids of C5-C18 as their 3,5-dinitrophenylurethane and methyl ester derivatives were achieved by high-performance liquid chromatography with a slurry-packed fused-silica capillary column (40 cm × 0.32 mm i.d.) with a chiral phase, N-(S)-2-(4-chlorophenyl)isovaleroyl-d-phenylglycine ionically bonded to 5-μm silica gel. The analysis was optimized by using a ternary mobile phase, n-hexane-1,2-dichloroethaneethanol, and UV detection at 226 nm.This publication has 0 references indexed in Scilit: