Antibodies of Different Specificities in Normal Rabbit Sera

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Abstract
Low levels of so-called “natural” antibodies have been reported in many studies (for review, see Boyden (1)). Recently, antibodies to defined haptens, such as the 2,4-dinitrophenyl (DNP) (2, 3), the 4-hydroxy-5-nitrophenylacetyl (4) and the penicilloyl (5) groups have been found in normal humans, mice and rabbits. In the course of experiments making use of the detection of antibodies by means of chemically modified bacteriophages (4–7), we have tested the sera of 28 normal rabbits for their capacity to inactivate immunospecifically modified bacteriophages of different specificities. The bacteriophage preparations used included DNP-T4 (3), penicilloyl-T4 (5), lysozyme-T4 (8), insulin-T4 (8), and the unmodified bacteriophage T4 itself. The results obtained with sera pooled from the bleedings of 28 normal rabbits are given in Table I. The pooled rabbit sera, tested at fivefold dilution, inactivated almost completely DNP-T4, and decreased significantly the number of viable penicilloyl-T4, whereas no significant inactivation of insulin-T4, lysozyme-T4 and the unmodified T4 was observed.

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