Use of Leukocyte Chemotaxis in Vitro to Assay Mediators Generated by Immune Reactions

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Abstract
Polycarbonate (Nuclepore) filters were used to develop a rapid, reproducible technique to measure simultaneously mononuclear and polymorphonuclear leukocyte chemotaxis in vitro. Both cell types, obtained from guinea pig peritoneal exudates, showed a chemotactic response to an Escherichia coli filtrate. Neutrophils showed a faster, stronger response than did mononuclear cells and seemed to inhibit mononuclear chemotactic activity. Peritoneal exudate stored for 4 days did not lose its chemotactic responsiveness. This technique may be used to detect chemotactic activity generated by humoral and cellular immune reactions and to study the relative responsiveness of various leukocytes to the same agent.

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