A rapid method for the preparation of high potency auto and alloantibody eluates

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Abstract
This report describes a modified, rapid acid-stromal method for elution of [human] erythrocyte antibodies. With this method potent crystal clear auto antibody and alloantibody eluates can be produced. The simplicity of the method makes it readily adaptable to the routine serological laboratory or blood bank. In semiquantitative and quantitative studies this method compares favorably with other antibody elution methods. With alloantibodies the rapid acid elution method and the ether method produced the strongest eluates, with the ether eluates being slightly more potent than the rapid eluates. With autoantibodies the rapid method was clearly superior, producing much stronger Ig[immunoglobulin]G erythrocyte coating as judged by the 125I antiglobulin test.