Chlorophyll Biosynthesis in a Cell-free System from Higher Plants
- 1 January 1971
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 47 (1) , 33-37
- https://doi.org/10.1104/pp.47.1.33
Abstract
A crude homogenate obtained from greening cucumber (Cucumis sativus, L.) cotyledons in tris-sucrose, pH 7.7, containing coenzyme A, glutathione, potassium phosphate, pH 7.7, methyl alcohol, magnesium, nicotinamide adenine dinucleotide, and adenosine triphosphate, is able to incorporate 4-(14)C-delta-aminolevulinic acid into chlorophyll a and b in the presence of oxygen. If the homogenates are prepared from etiolated cotyledons which have been exposed to light for two and one-half hours, (14)C-chlorophyll a is synthesized. However, when the homogenates are prepared from cotyledons illuminated for four and one-half hours, both (14)C-chlorophyll a and b are produced.Keywords
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