Chiasma Frequency and Disjunctional Behavior of Ewe and Cow Oocytes Matured In Vitro

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Abstract
Oocytes obtained from adult female sheep and cattle were studied during in vitro meiotic maturation for cytogenetic data. Ninety-three diakinesis/first-metaphase ewe preparations obtained afer 16-24 hr of culture had an average chiasma frequency per bivalent of 1.30. One hundred and seventy-seven ewe second-metaphase ova obtained at 23-48 hr of culture contained 27 symmetrical chromosomes. One possible heterozygote was seen at second metaphase, but was not reflected in appropriate first-metaphase configurations. One hundred and nine diakinesis/first-metaphase cow preparations obtained after 8-26 hr of culture had an average chiasma frequency per bivalent of 1.19. Ninety-nine cow second-metaphase ova obtained at 19-48 hr of culture contained 30 chromosomes plus 3 which were diploid. Comparisons with in vivo findings for these species and in vitro data for other species are made.

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