BASE SEQUENCE DETERMINATION IN NUCLEIC ACIDS WITH THE ELECTRON MICROSCOPE, III. CHEMISTRY AND MICROSCOPY OF GUANINE-LABELED DNA
- 1 March 1965
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 53 (3) , 564-571
- https://doi.org/10.1073/pnas.53.3.564
Abstract
Diazotized 2-amino-p-benzenedisulphonic acid can selectively label the guanine residues in DNA. The reaction is mild and no excessive degradation of the polymer chain is observed, DNA so labeled and stained with uranyl acetate is visible in the electron microscope and the markers resolved in pairs of dots 10-12 A apart. Pairs have been observed spaced 6-7 A from each other, suggesting labeling of consecutive guanine residues.Keywords
This publication has 5 references indexed in Scilit:
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