On the Stereospecificity of 26-Hydroxylation of Cholesterol. Bile Acid and Steroids 155.

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Abstract
The stereospecificity of the 26-hydroxylation of cholesterol in mouse liver homogenates was studied. 14C -Cholesterol labeled inter alia in one of the two terminal methyl groups of the side-chain was converted enzymically into cholest-5-ene-3[beta], 26-diol. This sterol was oxidized to 3-keto-5[alpha]-cholestanoic acid which was decarboxylated. The isotope content of the carbon dioxide liberated was such as to demonstrate that the 26-hydroxylation occurs in a stereospecific manner.