EFFECTS OF NICKEL CHLORIDE AND DIETHYLDITHIOCARBAMATE ON METALLOTHIONEIN IN RAT-LIVER AND KIDNEY

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  • 1 January 1983
    • journal article
    • research article
    • Vol. 13  (6) , 489-495
Abstract
Effects of NiCl2 and sodium diethyldithiocarbamate (DDC) upon metallothionein (MT) concentrations were studied in liver and kidney of male Fischer rats. After injection of NiCl2 (0.75 mmol/kg, s.c.), hepatic MT concentration increased 2.6-fold at 6.5 h and 8.2-fold at 17 h; renal MT concentration increased 1.4-fold at 6.5 h and 2.3-fold at 17 h. Dose-related increases of MT concentrations were observed in liver and kidney of rats killed 17 h after injection of NiCl2 (0.25-0.75 mmol/kg, s.c.). Repeated administration of NiCl2 (0.1 mmol/kg i.p.) on 4 successive days, with sacrifice 3 days after the last treatment, increased MT concentrations 1.4-fold in liver and kidney, whereas CdCl2-treatment at the same dosage schedule increased MT concentration 16-fold in liver and 3.3-fold in kidney. NiCl2-Induction of MT in liver and kidney was not prevented by actinomycin D (1 mg/kg, i.p.), but was inhibited by cycloheximide (2 mg/kg, i.p.). Sodium diethyldithiocarbamate given alone (1.33 mmol/kg, i.m.) 17 h before death, increased MT concentration 7.6-fold in liver but did not affect MT concentration in kidney; administration of DDC prior to injection of NiCl2 did not inhibit NiCl2-induction of MT.