Sensitivity of PCR in detecting monoclonal B cell proliferations.
Open Access
- 1 July 1993
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 46 (7) , 624-627
- https://doi.org/10.1136/jcp.46.7.624
Abstract
AIMS--To evaluate the rapid detection of various forms of monoclonal B cell proliferations by using the polymerase chain reaction (PCR) to identify clonal immunoglobulin heavy chain genomic rearrangements. METHODS--Thirty four B cell lymphomas defined by morphology, immunophenotyping, and positive immunoglobulin heavy chain gene rearrangements detected by Southern blot analysis were examined. An additional 22 cases representing miscellaneous lymphoproliferative and non-lymphoproliferative disorders were also studied. RESULTS--Monoclonal rearrangements were identified in 19 (56%) cases of B cell lymphoma. The method was less sensitive in the detection of follicular centre cell lymphomas (15 of 28, or 54%) than non-follicular centre cell lesions (four of six, or 67%). Monoclonal rearrangement was not identified in 19 control cases, including T cell lymphomas, Hodgkin's disease, reactive lymphadenopathy and metastatic carcinoma. Three cases showed positive immunoglobulin gene rearrangement by PCR but were negative on Southern blotting. Two of these cases had definite clinical, morphological, and immunophenotypic evidence of monoclonal B cell proliferation suggesting that PCR could, on occasion, pick up cases missed by Southern blotting and that the two methods are complementary in clonal lymphoproliferative disease diagnosis. The third case represented a "false positive" PCR reaction involving a colonic adenocarcinoma. CONCLUSIONS--PCR analysis, using the primer sequences outlined in this study, will detect about 55% of clonal lymphoproliferative proliferations with increased sensitivity for non-follicular centre cell lesions. With these levels of detection in mind, this testing strategy can still be especially useful in cases which prove diagnostically problematic with standard morphological and immunophenotypic analysis, and in instances where the quantity and type of diagnostic material is limiting (needle aspirates and cellular fluids).Keywords
This publication has 19 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Improved PCR method for detecting monoclonal immunoglobulin heavy chain rearrangement in B cell neoplasms.Journal of Clinical Pathology, 1992
- Monoclonality in B cell lymphoma detected in paraffin wax embedded sections using the polymerase chain reaction.Journal of Clinical Pathology, 1990
- Rapid method for distinguishing clonal from polyclonal B cell populations in surgical biopsy specimens.Journal of Clinical Pathology, 1990
- The Evolution of B-Cell ClonesPublished by Springer Nature ,1990
- Avoiding false positives with PCRNature, 1989
- Single cell origin of bigenotypic and biphenotypic B cell proliferations in human follicular lymphomas.The Journal of Experimental Medicine, 1988
- Antigen‐Driven Selection of Virgin and Memory B CellsImmunological Reviews, 1986
- Immunoglobulin gene rearrangement as a diagnostic criterion of B-cell lymphoma.Proceedings of the National Academy of Sciences, 1984
- Immunoglobulin-Gene Rearrangements as Unique Clonal Markers in Human Lymphoid NeoplasmsNew England Journal of Medicine, 1983