Binding of rat ribosomal proteins to yeast 5.8S ribosomal ribonucleic acid
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (7) , 2199-2207
- https://doi.org/10.1093/nar/10.7.2199
Abstract
5.8 S RNA-protein complexes were prepared using purified yeast 5.8 S RNA and proteins from the large ribosomal subunit of rat liver. Formation of such hybrid complexes, as measured by Millipore filtration, was dependent on protein concentration. Binding of proteins to the RNA could approach saturation. Such complexes were isolated from sucrose density gradient centrifugation and shown to contain proteins L6, L8, L19, L35 and L35a. These proteins were identified by their molecular weights on polyacrylamide gels containing dodecylsulfate and their mobilities on two dimensional polyacrylamide gels.Keywords
This publication has 24 references indexed in Scilit:
- The Nucleotide Sequence of Saccharomyces cerevisiae 5.8 S Ribosomal Ribonucleic AcidJournal of Biological Chemistry, 1973
- Ribosomal RNA synthesis in Saccharomyces cerevisiaeJournal of Molecular Biology, 1972
- Reconstitution of 50S Ribosomal Subunits from Dissociated Molecular ComponentsNature, 1970
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Ribosomal proteins. VIIAnalytical Biochemistry, 1970
- Active hybrid 80 s particles formed from subunits of rat, rabbit and protozoan (Tetrahymena pyriformis) ribosomesJournal of Molecular Biology, 1969
- Ribosomal proteins of Escherichia coli. I. Purification of the 30 S ribosomal proteinsBiochemistry, 1969
- Structure and function of Escherichia coli ribosomes: VI. Mechanism of assembly of 30 s ribosomes studied in vitroJournal of Molecular Biology, 1969
- Characterization of a new low molecular weight RNA in HeLa cell ribosomesJournal of Molecular Biology, 1968
- A two-dimensional fractionation procedure for radioactive nucleotidesJournal of Molecular Biology, 1965