Uptake of l‐[35S]Cystine by Isolated Rat Brain Capillaries
- 1 August 1980
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 35 (2) , 417-424
- https://doi.org/10.1111/j.1471-4159.1980.tb06281.x
Abstract
Adult rat brain capillaries were isolated by a simplified procedure and showed an enrichment of the marker enzyme, γ‐glutamyltranspeptidase. The uptake of [35S]cystine at 37°C by this preparation can be divided into two components, a sodium‐ and energy‐dependent transport process for the free amino acid pool, with an apparent Km of 36 μm, and a binding process, with an apparent Km of 1.13 mm. Chemical analysis of the amino acid pool indicates that cystine is the major form of intracapillary 35S. Cystine transport was not inhibited by lysine, but glycine, α‐methylaminoisobutyric acid and β‐2‐aminobicyclo‐[2,2,1]‐heptane‐2‐carboxylic acid were inhibitory to a small extent.Keywords
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