Domain Interaction Between NMDA Receptor Subunits and the Postsynaptic Density Protein PSD-95
- 22 September 1995
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 269 (5231) , 1737-1740
- https://doi.org/10.1126/science.7569905
Abstract
The N-methyl-D-aspartate (NMDA) receptor subserves synaptic glutamate-induced transmission and plasticity in central neurons. The yeast two-hybrid system was used to show that the cytoplasmic tails of NMDA receptor subunits interact with a prominent postsynaptic density protein PSD-95. The second PDZ domain in PSD-95 binds to the seven-amino acid, COOH-terminal domain containing the terminal tSXV motif (where S is serine, X is any amino acid, and V is valine) common to NR2 subunits and certain NR1 splice forms. Transcripts encoding PSD-95 are expressed in a pattern similar to that of NMDA receptors, and the NR2B subunit co-localizes with PSD-95 in cultured rat hippocampal neurons. The interaction of these proteins may affect the plasticity of excitatory synapses.Keywords
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