The Mung Bean Rooting Bioassay: A Re-examination1

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Abstract
Inconsistent results obtained with the mung bean (Phaseolus aureus Roxb.) rooting bioassay led to a reexamination of procedures. Autoclaving the double distilled water used completely eliminated the inconsistent results, but boiling and filter sterilization were not completely satisfactory. A decrease in rooting of both control and auxin-treated cuttings was noted in seedlings older than 10 and 9 days respectively. Adventitious roots were initiated within 5 days; incubation for 2 additional days did not increase rooting response. Increasing irradiance from 380 to 4080 μW/cm2 decreased rooting of both control and auxin treated cuttings.

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