A universal post-embedding protocol for immunogold labelling of osmium-fixed, epoxy resin-embedded tissue
- 1 January 1997
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Electron Microscopy
- Vol. 46 (4) , 315-319
- https://doi.org/10.1093/oxfordjournals.jmicro.a023525
Abstract
The authors have found that double etching of epoxy-embedded, ultrathin sections with 3% sodium metaperiodate rendered epitope expression comparable to that obtained with either saturated or half-saturated sodium metaperiodate solutions. In contrast to either of the two more concentrated oxidizing solutions, double etching with 3% sodium metaperiodate neither bleached the specimens nor generated holes in the plastic resin.Keywords
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