A soluble chloroplast protein catalyzes ribulosebisphosphate carboxylase/oxygenase activation in vivo
- 1 January 1985
- journal article
- research article
- Published by Springer Nature in Photosynthesis Research
- Vol. 7 (2) , 193-201
- https://doi.org/10.1007/bf00037012
Abstract
Ribulosebisphosphate carboxylase/oxygenase (EC 4.1.1.39) (rubisco) must be fully activated in order to catalyze the maximum rates of photosynthesis observed in plants. Activation of the isolated enzyme occurs spontaneously, but conditions required to observe full activation are inconsistent with those known to occur in illuminated chloroplasts. Genetic studies with a nutant of Arabidopsis thaliana incapable of activating rubisco linked two chloroplast polypeptides to the activation process in vivo. Using a reconstituted light activation system, it was possible to demonstrate the participation of a chloroplast protein in rubisco activation. These results indicate that a specific chloroplast enzyme, rubisco activase, catalyzes the activation of rubisco in vivo.Keywords
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