The Effects of Irradiation on Endocrine Cells: V. A Comparison between the Effects of Inanition and Irradiation on Androgen Production by Murine Testicular Tissue

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Abstract
Male rats (Holtzman strain) were placed on a restricted diet of 7.5 gms of laboratory chow daily for 20 days. The control group received the same ration ad libitum, and water was given to both groups ad libitum. Testicular tissue from both groups was incubated with 5-pregnenolone-7alpha-H3 and progesterone-4-C14 , made with and without added cofactors. After 20 days on the restricted diet, body weight, testicular weight, and seminal vesicle weight decreased and in vitro biosynthesis of androgens was markedly diminished. The effects of inanition on androgen biosynthesis differed from those observed previously for irradiation in that they were not reversed by the addition of a NADPH-generating system.